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Sample GSM630219 Query DataSets for GSM630219
Status Public on Dec 09, 2010
Title pbs2-del-2-a
Sample type RNA
 
Channel 1
Source name refpool
Organism Saccharomyces cerevisiae
Characteristics strain (or line): BY4742
genotype/variation: refpool
Extracted molecule total RNA
Extraction protocol yeast RNA isolation for robotic amplification v1.0
Label Cy5
Label protocol robot amplification and labeling v1.0
 
Channel 2
Source name pbs2-del
Organism Saccharomyces cerevisiae
Characteristics strain (or line): BY4742
genotype/variation: pbs2-del
Extracted molecule total RNA
Extraction protocol yeast RNA isolation for robotic amplification v1.0
Label Cy3
Label protocol robot amplification and labeling v1.0
 
 
Hybridization protocol Tecan HS4800 hybridization
Scan protocol scanning of slides using Agilent G256BA
Features were extracted using Imagene software from Biodiscovery.
Data processing genes, no bg corr, dye corr: as genes, no bg corr, but followed by gene-specific dye bias correction. Identical to genes, no bg corr if the dye bias correction is 0.0, which is the default. So far (jan 2008), we have only dye-bias corrections for yeast.
limma: A software package for the analysis of gene expression microarray data, especially the use of linear models for analysing designed experiments and the assessment of differential expression. Author(s): Gordon Smyth. The limma R package version 2.12.0 is used. P-values are Benjamini-Hochberg FDR corrected.
 
Submission date Nov 29, 2010
Last update date Jun 06, 2013
Contact name Patrick Kemmeren
Organization name UMC Utrecht
Department Department of Molecular Cancer Research
Lab Holstege Lab
Street address Universiteitsweg 100
City Utrecht
State/province Utrecht
ZIP/Postal code 3584 CG
Country Netherlands
 
Platform ID GPL11232
Series (3)
GSE25644 Expression profiling of yeast kinase and phosphatase knockouts
GSE42527 Compendium of deletion mutant gene expression profiles, 700 responsive mutants [hs1991]
GSE42528 Compendium of deletion mutant gene expression profiles

Data table header descriptions
ID_REF
VALUE normalized log2 ratio test/ref
Signal Norm_Cy5
Signal Norm_Cy3

Data table
ID_REF VALUE Signal Norm_Cy5 Signal Norm_Cy3
1 0.375520405160675 7493.96 9721.97
2 -0.0477581795363132 258.311 249.9
3 0.0799757808332822 526.892 556.925
4 0.0414570214340675 49.7896 51.2411
5 -0.156920310682685 537.015 481.669
6 -0.0359871906849968 124.615 121.545
7 -0.0167062808121575 462.342 457.019
8 0.0265646699611492 971.653 989.71
9 0.102601187591769 349.394 375.147
10 0.0859961679440164 56.5366 60.0091
11 0.203572331976562 3783.07 4356.38
12 0.0523454184771935 49.4569 51.2843
13 0.074707014544884 146.8 154.602
14 -0.173908671466016 170.356 151.01
15 -0.0628001696611635 2822.1 2701.89
16 0.0516874232080399 69.3926 71.9238
17 0.0523074008698024 49.7183 51.554
18 0.353111249280385 8483.83 10836.5
19 -0.171988291061393 11283 10015
20 -0.130945225625163 986.872 901.244

Total number of rows: 15552

Table truncated, full table size 609 Kbytes.




Supplementary file Size Download File type/resource
GSM630219.txt.gz 673.3 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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