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Sample GSM630177 Query DataSets for GSM630177
Status Public on Dec 09, 2010
Title kin82-del-1-a
Sample type RNA
 
Channel 1
Source name refpool
Organism Saccharomyces cerevisiae
Characteristics strain (or line): BY4742
genotype/variation: refpool
Extracted molecule total RNA
Extraction protocol yeast RNA isolation for robotic amplification v1.0
Label Cy5
Label protocol robot amplification and labeling v1.0
 
Channel 2
Source name kin82-del
Organism Saccharomyces cerevisiae
Characteristics strain (or line): BY4742
genotype/variation: kin82-del
Extracted molecule total RNA
Extraction protocol yeast RNA isolation for robotic amplification v1.0
Label Cy3
Label protocol robot amplification and labeling v1.0
 
 
Hybridization protocol Tecan HS4800 hybridization
Scan protocol scanning of slides using Agilent G256BA
Features were extracted using Imagene software from Biodiscovery.
Data processing genes, no bg corr, dye corr: as genes, no bg corr, but followed by gene-specific dye bias correction. Identical to genes, no bg corr if the dye bias correction is 0.0, which is the default. So far (jan 2008), we have only dye-bias corrections for yeast.
limma: A software package for the analysis of gene expression microarray data, especially the use of linear models for analysing designed experiments and the assessment of differential expression. Author(s): Gordon Smyth. The limma R package version 2.12.0 is used. P-values are Benjamini-Hochberg FDR corrected.
 
Submission date Nov 29, 2010
Last update date Jun 06, 2013
Contact name Patrick Kemmeren
Organization name UMC Utrecht
Department Department of Molecular Cancer Research
Lab Holstege Lab
Street address Universiteitsweg 100
City Utrecht
State/province Utrecht
ZIP/Postal code 3584 CG
Country Netherlands
 
Platform ID GPL11232
Series (3)
GSE25644 Expression profiling of yeast kinase and phosphatase knockouts
GSE42527 Compendium of deletion mutant gene expression profiles, 700 responsive mutants [hs1991]
GSE42528 Compendium of deletion mutant gene expression profiles

Data table header descriptions
ID_REF
VALUE normalized log2 ratio test/ref
Signal Norm_Cy5
Signal Norm_Cy3

Data table
ID_REF VALUE Signal Norm_Cy5 Signal Norm_Cy3
1 -0.171064625932996 35048.7 31129.8
2 -0.172737360833612 474.716 421.148
3 -0.0268255101237764 1190.41 1168.48
4 -0.128492586113843 57.2728 52.3924
5 -0.0571820837564749 1194.79 1148.36
6 -0.118539363693988 301.102 277.351
7 0.0533813739845345 1110.25 1152.1
8 0.0563719047585533 2564.53 2666.72
9 -0.0202951229026821 806.642 795.374
10 0.0882305734914207 67.433 71.6857
11 0.0369262030680542 9109.12 9345.28
12 -0.0768872955392696 60.3037 57.174
13 -0.116590500956873 453.197 418.013
14 0.0496649748557484 356.55 369.038
15 -0.0146314719047203 7204.79 7132.09
16 0.00329906798579422 93.6071 93.8214
17 -0.090579745197585 59.458 55.8397
18 -0.153584463094931 43490.2 39098.3
19 -0.0163576250529086 28081.8 27765.2
20 -0.114277235786411 1920.82 1774.54

Total number of rows: 15552

Table truncated, full table size 610 Kbytes.




Supplementary file Size Download File type/resource
GSM630177.txt.gz 658.9 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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