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Sample GSM630136 Query DataSets for GSM630136
Status Public on Dec 09, 2010
Title elm1-del-3-b
Sample type RNA
 
Channel 1
Source name elm1-del
Organism Saccharomyces cerevisiae
Characteristics strain (or line): BY4742
genotype/variation: elm1-del
Extracted molecule total RNA
Extraction protocol yeast RNA isolation for robotic amplification v1.0
Label Cy5
Label protocol robot amplification and labeling v1.0
 
Channel 2
Source name refpool
Organism Saccharomyces cerevisiae
Characteristics strain (or line): BY4742
genotype/variation: refpool
Extracted molecule total RNA
Extraction protocol yeast RNA isolation for robotic amplification v1.0
Label Cy3
Label protocol robot amplification and labeling v1.0
 
 
Hybridization protocol Tecan HS4800 hybridization
Scan protocol scanning of slides using Agilent G256BA
Features were extracted using Imagene software from Biodiscovery.
Data processing genes, no bg corr, dye corr: as genes, no bg corr, but followed by gene-specific dye bias correction. Identical to genes, no bg corr if the dye bias correction is 0.0, which is the default. So far (jan 2008), we have only dye-bias corrections for yeast.
limma: A software package for the analysis of gene expression microarray data, especially the use of linear models for analysing designed experiments and the assessment of differential expression. Author(s): Gordon Smyth. The limma R package version 2.12.0 is used. P-values are Benjamini-Hochberg FDR corrected.
 
Submission date Nov 29, 2010
Last update date Jun 06, 2013
Contact name Patrick Kemmeren
Organization name UMC Utrecht
Department Department of Molecular Cancer Research
Lab Holstege Lab
Street address Universiteitsweg 100
City Utrecht
State/province Utrecht
ZIP/Postal code 3584 CG
Country Netherlands
 
Platform ID GPL11232
Series (5)
GSE25644 Expression profiling of yeast kinase and phosphatase knockouts
GSE33097 Deletion mutant analysis of established glucose signaling and metabolic pathway members in Saccharomyces cerevisiae
GSE33099 Glucose signaling

Data table header descriptions
ID_REF
VALUE normalized log2 ratio test/ref
Signal Norm_Cy5
Signal Norm_Cy3

Data table
ID_REF VALUE Signal Norm_Cy5 Signal Norm_Cy3
1 -0.266392080868898 3387.59 4074.58
2 0.0666220886538678 283.765 270.959
3 0.021794726117724 585.457 576.679
4 -0.0542380634943126 52.7948 54.8174
5 -0.0824590139438883 560.444 593.41
6 -0.0111148961372558 126.713 127.693
7 -0.143305854707153 445.616 492.153
8 0.130917337436841 989.874 904.003
9 0.145162123495274 400.701 362.345
10 -0.0683598800883777 59.2614 62.137
11 0.316176779145037 4560.06 3662.62
12 -0.028544505604014 53.1413 54.2032
13 -0.0697458877635128 162.219 170.254
14 0.106994166584051 203.793 189.226
15 0.0372475375431663 2784.1 2713.14
16 -0.074770314433923 74.4078 78.3658
17 -0.0423170921010573 53.7183 55.3173
18 -0.13126036542333 7448.39 8157.85
19 -0.123017652320992 10667.3 11616.8
20 -0.0719872173009147 947.881 996.378

Total number of rows: 15552

Table truncated, full table size 608 Kbytes.




Supplementary file Size Download File type/resource
GSM630136.txt.gz 677.6 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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