|
| Status |
Public on Nov 24, 2010 |
| Title |
Postnatal_23 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Piedmontese sample from 25 months old
|
| Organism |
Bos taurus |
| Characteristics |
sample: GP130
treatment: Piedmontese sample from 25 months old
tissue: longissimus dorsi skeletal muscle biopsy
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Muscle samples of 1 to 2 g were removed using a scalpel and immediately snap-frozen using liquid nitrogen for RNA analyses. Total RNA was prepared from each sample biopsy using TRIzol reagent(Invitrogen) in accordance with the manufacturers protocol.
|
| Label |
Cy3
|
| Label protocol |
Amplified and labeled according to Agilent Two-Color Microarray-Based Gene Expression Analysis protocol
|
| |
|
| Channel 2 |
| Source name |
Wagyu sample from 25 months old
|
| Organism |
Bos taurus |
| Characteristics |
sample: GW169
treatment: Wagyu sample from 25 months old
tissue: longissimus dorsi skeletal muscle biopsy
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Muscle samples of 1 to 2 g were removed using a scalpel and immediately snap-frozen using liquid nitrogen for RNA analyses. Total RNA was prepared from each sample biopsy using TRIzol reagent(Invitrogen) in accordance with the manufacturers protocol.
|
| Label |
Cy5
|
| Label protocol |
Amplified and labeled according to Agilent Two-Color Microarray-Based Gene Expression Analysis protocol
|
| |
|
| |
| Hybridization protocol |
Hybridized according to Agilent Two-Color Microarray_based Gene Expression Analysis protocol
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner.
|
| Description |
251535410121-3
|
| Data processing |
Images were quantified using Agilent Feature Extraction software (Version 9.5.1)
|
| |
|
| Submission date |
Nov 23, 2010 |
| Last update date |
Nov 23, 2010 |
| Contact name |
Sean McWilliam |
| E-mail(s) |
sean.mcwilliam@csiro.au
|
| Organization name |
CSIRO
|
| Department |
Agriculture and Food
|
| Street address |
306 Carmody Road
|
| City |
St Lucia |
| State/province |
Queensland |
| ZIP/Postal code |
4067 |
| Country |
Australia |
| |
|
| Platform ID |
GPL7053 |
| Series (1) |
| GSE25554 |
The Transcriptional Landscape of Bovine Skeletal Muscle |
|
