|
| Status |
Public on Dec 01, 2011 |
| Title |
RSG_12h_rep5 |
| Sample type |
RNA |
| |
|
| Source name |
THP-1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: THP-1
differentiation: PMA-differentiated THP-1 cells
treatment: RSG (1microM)
time (hrs): 12
|
| Treatment protocol |
THP-1 cells were differentiated with PMA (10ng/ml) over the course of 40h. Cells were washed and incubated with medium w/o PMA for 2 hours prior to stimulation.
|
| Growth protocol |
THP-1 cells (ATCC TIB-202) were maintained in RPMI-1640 medium supplemented with 10% FBS at 37C and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAGEN RNeasy mini kit following the standard protocol provided with the extraction kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion totalRNA kit for Illumina
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 5
|
| Data processing |
The data were normalised using average normalisation with BeadsStudio 3.1.3.0 (Illumina)
|
| |
|
| Submission date |
Nov 04, 2010 |
| Last update date |
Dec 01, 2011 |
| Contact name |
Sebastian Pott |
| E-mail(s) |
spott@uchicago.edu
|
| Organization name |
University of Chicago
|
| Department |
Human Genetics
|
| Street address |
920 58th Street
|
| City |
Chicago |
| State/province |
Il |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL6883 |
| Series (2) |
| GSE25137 |
Functional and cellular constraints that shaped the PPARg binding landscape in human and mouse macrophages: human expression |
| GSE25608 |
Functional and cellular constraints that shaped the PPARg binding landscape in human and mouse macrophages |
|
