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| Status |
Public on Dec 01, 2011 |
| Title |
Functional and cellular constraints that shaped the PPARg binding landscape in human and mouse macrophages: human expression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Genome-wide comparisons of transcription factor binding sites in different species allow for a direct evaluation of the evolutionary constraints that shape transcription factor binding landscapes. To gain insights into the evolution of the PPARg-dependent transcriptional network we obtained binding data for PPARg, RXR and PU.1 in human macrophages and compared the profiles to matching data from mouse macrophages. We found that PPARg binding was highly divergent and only 5% of the PPARg bound regions were occupied in both species. Despite the low conservation of PPARg binding sites, conserved PPARg target genes contribute more than 30% to the functional target genes identified in human macrophages. In addition conserved target genes are strongly enriched for lipid metabolic functions. We detected the lineage-specification factor PU.1 at the majority of human PPARg binding sites. This confirmed the juxtaposed binding configuration found in mouse macrophages and demonstrated the preservation of tissue-specific adjacent PPARg-Pu.1 binding in the absence of individual binding site conservation. Finally, based on this of PPARg and PU.1 binding between human and mouse we suggest a mechanism by which PU.1 facilitates PPARg binding site turnover in macrophages.
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| |
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| Overall design |
Comparison of RNAs level in Rosiglitazone (RSG) and vehicle (DMSO) treated PMA differentiated THP-1 cells over a timecourse from 0.5h to 12h
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| |
|
| Contributor(s) |
Liu ET |
| Citation(s) |
23118933 |
| Submission date |
Nov 04, 2010 |
| Last update date |
Aug 18, 2017 |
| Contact name |
Sebastian Pott |
| E-mail(s) |
spott@uchicago.edu
|
| Organization name |
University of Chicago
|
| Department |
Human Genetics
|
| Street address |
920 58th Street
|
| City |
Chicago |
| State/province |
Il |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6883 |
Illumina HumanRef-8 v3.0 expression beadchip |
|
| Samples (59)
|
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| This SubSeries is part of SuperSeries: |
| GSE25608 |
Functional and cellular constraints that shaped the PPARg binding landscape in human and mouse macrophages |
|
| Relations |
| BioProject |
PRJNA142767 |
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