|
| Status |
Public on Jan 10, 2011 |
| Title |
siERK1E24h_2651 |
| Sample type |
RNA |
| |
|
| Source name |
MCF-7 cells, siERK1 treatment 60h, 10 nM E2 for 4h
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Breast adenocarcinoma
cell line: MCF-7
sirna: siERK1
agent: E2
time: 4h
|
| Treatment protocol |
MCF-7 human breast cancer cells were transfected with 20 nM of siGENOME ctrl (siGL3),ERK1 or ERK2 (Thermo Scientific) using Dharmafect1 following the manufacturer’s instructions. After 60 h of transfection, cells were treated with 0.1% control ethanol vehicle or 10 nM E2 for 4h or 24h
|
| Growth protocol |
MCF-7 cells were maintained in MEM media with 5% Calf serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner GCS 3000.
|
| Description |
2651_E_18_(HG-U133A_2).CEL
Gene expression data from MCF-7 cells, siERK1 treatment 60h, 10 nM E2 for 4h
|
| Data processing |
The data were analyzed with GCOS2.1 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Oct 08, 2010 |
| Last update date |
Jan 10, 2011 |
| Contact name |
Zeynep Madak Erdogan |
| Organization name |
UIUC
|
| Department |
FSHN
|
| Lab |
Zeynep Madak Erdogan
|
| Street address |
1201 S Goodwin Ave
|
| City |
Urbana |
| State/province |
IL |
| ZIP/Postal code |
61801 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE24592 |
Genomic Collaboration of Estrogen Receptor-α and ERK2 in Regulating Gene and Proliferation Programs |
|
