|
| Status |
Public on Dec 31, 2010 |
| Title |
tumor cells from bladder bU30 |
| Sample type |
RNA |
| |
|
| Source name |
urothelial cell carcinoma from bladder
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: urothelial cell carcinoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
10 ug of Qiagen-purified total RNA was labeled with 6ul of 1mg/ml R-Phycoerythrin Streptavidin (SAPE)
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
|
| Description |
tumor cells from bladder
|
| Data processing |
The data were analyzed with custom CDF environments created by the MBNI - The version 6 entrez gene ID CDF was used. Data were then rma normalized using R and the bioconductor suite of analysis tools (eset <- rma(ab))
|
| |
|
| Submission date |
Sep 15, 2010 |
| Last update date |
Dec 31, 2010 |
| Contact name |
Bin Tean Teh |
| Organization name |
Van Andel Institute
|
| Department |
Cancer Genetics
|
| Street address |
330 Bostwick Av
|
| City |
Grand Rapids |
| State/province |
MI |
| ZIP/Postal code |
49503 |
| Country |
USA |
| |
|
| Platform ID |
GPL6791 |
| Series (1) |
| GSE24152 |
Comparative Gene Expression Profiling Analysis of Urothelial Carcinoma of Renal Pelvis and Bladder |
|
