|
Status |
Public on Feb 22, 2022 |
Title |
S06_16X |
Sample type |
RNA |
|
|
Source name |
BAL Fluid_TDMT422_total RNA_myeloid-derived suppressor cell
|
Organism |
Homo sapiens |
Characteristics |
tissue: BAL Fluid pre_alveolar macrophageplification: 16X patient condition: Tuberculosis cell type: myeloid-derived suppressor cell patient_id: TDMT422
|
Extracted molecule |
total RNA |
Extraction protocol |
Pre-alveolar macrophageplification protocol: cDNA were further subjected to either 16 or 20 pre-alveolar macrophageplification cycles using Fluidigm One Tube Prealveolar macrophagep Master Mix (Fluidigm PN 100-5580) RNEasy® Micro Kit (Qiagen, Germany)
|
Label |
N/A
|
Label protocol |
N/A
|
|
|
Hybridization protocol |
N/A
|
Scan protocol |
N/A
|
Description |
S06_16X
|
Data processing |
import into R and normalisation based on 3 reference genes using BioConductor packages readqPCR and normqPCR statistical analysis using Stan via the brms package
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|
|
Submission date |
Feb 21, 2022 |
Last update date |
Feb 22, 2022 |
Contact name |
Gian van der Spuy |
Organization name |
Stellenbosch University
|
Department |
Biomedical Sciences
|
Street address |
Francie van Zijl Dr
|
City |
Parow |
ZIP/Postal code |
7530 |
Country |
South Africa |
|
|
Platform ID |
GPL31965 |
Series (1) |
GSE197113 |
Targeted gene expression profiling of human myeloid cells from blood and lung compartments of patients with Tuberculosis and other lung diseases |
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