|
| Status |
Public on Jun 01, 2023 |
| Title |
nubiterrae_forest_early_07 |
| Sample type |
SRA |
| |
|
| Source name |
E12.5-13.5 embryonic tail
|
| Organism |
Peromyscus maniculatus nubiterrae |
| Characteristics |
Stage: E12.5-13.5
ecotype: forest
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
We extracted total RNA using the PicoPure RNA Isolation kit (ThermoFisher Scientific).
We constructed libraries using a PrepX PolyA kit on an Apollo 324 System
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Trimmed reads using Cutadapt 1.13
Mapped reads using STAR 2.5.3
Estimated transcript abundance with RSEM 1.3.0
Combined counts into matrix with Trinity 2.4.0 abundance_estimates_to_matrix.pl
Genome_build: GCA_003704035.3
Supplementary_files_format_and_content: Matrix table with abundances for every gene and every sample
|
| |
|
| Submission date |
Dec 20, 2021 |
| Last update date |
Jun 01, 2023 |
| Contact name |
Evan Kingsley |
| E-mail(s) |
evan_kingsley@hms.harvard.edu
|
| Organization name |
Harvard Medical School
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL31116 |
| Series (1) |
| GSE191280 |
RNA-seq profiling of tissue from deer mouse embryo tails |
|
| Relations |
| BioSample |
SAMN24247598 |
| SRA |
SRX13456607 |
