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Sample GSM5742962 Query DataSets for GSM5742962
Status Public on Jun 01, 2023
Title nubiterrae_forest_early_03
Sample type SRA
 
Source name E12.5-13.5 embryonic tail
Organism Peromyscus maniculatus nubiterrae
Characteristics Stage: E12.5-13.5
ecotype: forest
Extracted molecule polyA RNA
Extraction protocol We extracted total RNA using the PicoPure RNA Isolation kit (ThermoFisher Scientific).
We constructed libraries using a PrepX PolyA kit on an Apollo 324 System
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Data processing Trimmed reads using Cutadapt 1.13
Mapped reads using STAR 2.5.3
Estimated transcript abundance with RSEM 1.3.0
Combined counts into matrix with Trinity 2.4.0 abundance_estimates_to_matrix.pl
Genome_build: GCA_003704035.3
Supplementary_files_format_and_content: Matrix table with abundances for every gene and every sample
 
Submission date Dec 20, 2021
Last update date Jun 01, 2023
Contact name Evan Kingsley
E-mail(s) evan_kingsley@hms.harvard.edu
Organization name Harvard Medical School
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL31116
Series (1)
GSE191280 RNA-seq profiling of tissue from deer mouse embryo tails
Relations
BioSample SAMN24247602
SRA SRX13456603

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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