|
| Status |
Public on Jun 22, 2011 |
| Title |
FFPE_E(-)H(-)_1 |
| Sample type |
RNA |
| |
|
| Source name |
Breast cancer, FFPE, E-H-
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: breast cancer
age: 51
type: IDC
size: 1.6
grade: 3
positive node: 1(23)
er: -
pr: -
her2: -
rin: 1.7
|
| Treatment protocol |
Breast cancer specimens were formalin fixed and paraffin embedded.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the RecoverAll Total Nucleic acid kit followed by DNase I treatment. Quality control was performed with the Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Standard Illumina labelling protocol.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
4936527002_G
Formalin-fixed, paraffin-embedded tissue.
ER: estrogen receptor; PR: progesterone receptor; HER2: human epidermal growth factor receptor 2; RIN: RNA integrity number.
|
| Data processing |
Image data were processed by Illumina GenomeStudio software using background subtraction and quantile normalization. Log2 expression ratio of an intensity value to the average signal in all samples for each transcript was calculated.
|
| |
|
| Submission date |
Aug 02, 2010 |
| Last update date |
Jun 22, 2011 |
| Contact name |
Dong-Yu Wang |
| Organization name |
UHN-PMH-OCI
|
| Street address |
610 University Ave
|
| City |
Toronto |
| ZIP/Postal code |
M5G 2M9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL8432 |
| Series (2) |
| GSE23384 |
Gene profiling using archival formalin-fixed paraffin-embedded breast cancer specimens can generate informative microarray data: A comparison with matched fresh fine needle aspiration biopsy samples (FFPE samples) |
| GSE23386 |
Clinical relevance of DNA microarray analyses using archival formalin-fixed paraffin-embedded breast cancer specimens |
|
