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| Status |
Public on Jul 21, 2010 |
| Title |
H3K27me1 ChIP wild-type |
| Sample type |
SRA |
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| Source name |
leaves
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| Organism |
Arabidopsis thaliana |
| Characteristics |
strain: Columbia
genotype: wildtype
tissue: leaf
chip antibody: H3K27me1
chip antibody manufacturer: Upstate
chip antibody catalog #: 07-448
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| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from the sorted nuclei using the PicoPure DNA Extraction Kit (Molecular Devices, Sunnyvale, CA) according to the manufacturer’s recommendations. ChIP was performed as previously described (Zhang et al, 2009) Illumina libraries for genomic DNA extracted from sorted nuclei and ChIP samples were made following manufacturer instructions (Illumina).
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| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer II |
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| Data processing |
Alignment: Sequence reads were mapped to the Arabidopsis genome using SeqMap (Jiang and Wong, 2008). Uniquely mapping reads with two or fewer mismatches were retained.
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| Submission date |
Jul 19, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Hume Stroud |
| Organization name |
UT Southwestern
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| Street address |
5323 Harry Hines Blvd
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| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
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| Platform ID |
GPL9302 |
| Series (1) |
| GSE22411 |
Regulation of heterochromatic DNA replication by histone H3 lysine 27 methyltransferases |
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| Relations |
| SRA |
SRX038421 |
| BioSample |
SAMN00190952 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM567818_H3K27me1col.txt.gz |
8.4 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Processed data provided as supplementary file |
| Raw data are available in SRA |
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