|
| Status |
Public on Aug 30, 2022 |
| Title |
P7 No2 beta-blocker treated heart |
| Sample type |
SRA |
| |
|
| Source name |
Cardiac cells
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Heart
strain: C57BL/6
age: P7
cell type: Cardiac cells
treatment: beta-blocker for 7 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolation was performed by QIAGEN RNeasy mini kit
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Illumina Casava software used for basecalling.
Sequenced reads were trimmed for adaptor sequence.
Reads mapped to mm10 whole genome by Tophat with default options
Fragment Per Kilobase of exon per Megabase of library size (FPKM) were calculated using Cufflinks with default options.
Genome_build: mm10
Supplementary_files_format_and_content: Matrix table with FPKM for every gene
|
| |
|
| Submission date |
Oct 19, 2021 |
| Last update date |
Aug 30, 2022 |
| Contact name |
Mei Xin |
| E-mail(s) |
mei.xin@cchmc.org
|
| Phone |
5138033464
|
| Organization name |
Cincinnati Children's Hospital
|
| Department |
EHCB
|
| Lab |
Xin
|
| Street address |
3333 Burnet Avenue
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45249 |
| Country |
USA |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE186099 |
Gene expression changes in beta-blocker treated neonatal hearts |
|
| Relations |
| BioSample |
SAMN22402124 |
| SRA |
SRX12683210 |
