|
| Status |
Public on Sep 02, 2021 |
| Title |
A498 cl1c_(miRNA-4_0) |
| Sample type |
RNA |
| |
|
| Source name |
ccRCC A498 sunitinib resistantcell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A498
cell type: clear cell renal cell carcinoma (ccRCC)
drug resistance: sunitinib resistant
|
| Treatment protocol |
Sunitinib concentrations were increased incrementally by 0.5uM steps until the IC50 values reached >10uM
|
| Growth protocol |
cells were grown over several months either with sunitinib (two clones per cell line) or without (parental cell lines).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted for cells with Trizol using standard protocols
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
miRNA expression data from clone 1 of 498 sunitinib resistant ccRCC cell line
|
| Data processing |
RMA processed in affymetrix TAC (v.4.0.2)
|
| |
|
| Submission date |
Aug 31, 2021 |
| Last update date |
Sep 03, 2021 |
| Contact name |
charles lawrie |
| E-mail(s) |
charles.lawrie@biodonostia.org
|
| Phone |
0622080463
|
| Organization name |
Biodonostia Research Institute
|
| Department |
Oncology
|
| Street address |
Paseo Mikeletegi, 83- planta 3
|
| City |
Donsotia-San Sebastian |
| ZIP/Postal code |
20012 |
| Country |
Spain |
| |
|
| Platform ID |
GPL30571 |
| Series (1) |
| GSE183140 |
Integrated miRNA-genomic analysis of sunitinib resistance in clear cell renal cell carcinoma (CCRCC) cell lines |
|
