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| Status |
Public on Sep 02, 2021 |
| Title |
Integrated miRNA-genomic analysis of sunitinib resistance in clear cell renal cell carcinoma (CCRCC) cell lines |
| Platform organisms |
Homo sapiens; Rattus norvegicus |
| Sample organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
Non-coding RNA profiling by array
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| Summary |
clariom D and miRNA v4.0 microarray analyses of 786-O, A498 and Caki-1 cells suntinib resistant cell lines and originator cell lines
we used intergrated pathway network analysis to identify miRNA-target gene pairs involved in sunitinib resistance.
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| Overall design |
sunitinib resistant renal cell lines (786-O, A498 and Caki-1) were produced using increasing quantities of sunitinib over many weeks until lines were resistant to >10uM. Two clones for each cell lines were independently produced in this manner. Resistant cells and parental (sensitive) cells were harvested and RNA extracted. Affymetrix miRNA (v4.0) and gene (Clariom D) microarrays were carried out on all the cell lines in technical triplicates
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| Contributor(s) |
Lawrie C, Armesto M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 31, 2021 |
| Last update date |
Sep 04, 2021 |
| Contact name |
charles lawrie |
| E-mail(s) |
charles.lawrie@biodonostia.org
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| Phone |
0622080463
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| Organization name |
Biodonostia Research Institute
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| Department |
Oncology
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| Street address |
Paseo Mikeletegi, 83- planta 3
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| City |
Donsotia-San Sebastian |
| ZIP/Postal code |
20012 |
| Country |
Spain |
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| Platforms (2) |
| GPL23126 |
[Clariom_D_Human] Affymetrix Human Clariom D Assay [transcript (gene) version] |
| GPL30571 |
[miRNA-4] Affymetrix Rat miRNA-4 Array [Brain Array V21] |
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| Samples (54)
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| Relations |
| BioProject |
PRJNA759252 |
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