|
| Status |
Public on May 18, 2011 |
| Title |
35S:WIND1 callus replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Callus-like Arabidospsis thaliana expressing 35S:WIND1 with no treatment
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
strain: Col-0
genotype/variation: 35S:WIND1
treatment group: none
morphology: callus
tissue: callus
|
| Treatment protocol |
Mediums for T87 callus and 2,4-D-induced callus contain 500 nM NAA or 500 micro g/L 2,4-D to keep them callus state.
|
| Growth protocol |
Grown or cultured on solid MS medium with or without 500 nM NAA or 500 micro g/L 2,4-D.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol.
|
| Label |
Cy3
|
| Label protocol |
1 µg of total RNA were used for reverse transcription at 40°C for 2 h, and labeled cRNA were prepared using T7 RNA polymerase with Cy3-labeled CTP at 40°C for 2 h following manufacture's instructions (Agilent Quick Amp Labeling kit).
|
| |
|
| Hybridization protocol |
Fragmented 1.65 micro g labeled cRNAs with hybridization buffer (Agilent GE hybridization buffer HI-PRM) were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed.
|
| Scan protocol |
Images were quantified using Agilent Feature Extraction Software (version 9.1.3.1).
|
| Description |
Biological replicate (different callus) 2 of 3.
|
| Data processing |
Agilent Feature Extraction Software (v 9.1.3.1) was used for background subtraction.
Ln value of background-subtracted signal intensity (gProcessedSignal) divided by median value of filter-passed spots.
|
| |
|
| Submission date |
May 03, 2010 |
| Last update date |
May 18, 2011 |
| Contact name |
Nobutaka Mitsuda |
| E-mail(s) |
nobutaka.mitsuda@aist.go.jp
|
| Organization name |
National Institute of Advanced Industrial Science and Technology
|
| Department |
Bioproduction Research Institute
|
| Lab |
Gene Regulation Research Group
|
| Street address |
Central 6 Higashi 1-1-1
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8566 |
| Country |
Japan |
| |
|
| Platform ID |
GPL7299 |
| Series (1) |
| GSE21631 |
Comparison of Arabidopsis callus |
|
