|
| Status |
Public on May 15, 2021 |
| Title |
Bo-3 |
| Sample type |
SRA |
| |
|
| Source name |
passage 10 to 15 as aging group
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: vascular smooth muscle cells (VSMCs)
tissue source: blood vessel
treatment/sample group: Senescent
|
| Treatment protocol |
VMSCs of third to fifth generation cells were used as unaging cells (Bo-1, -2, -3), and cells treated with 50 nM Olmesartan was set as treated cells (Bo-ao-1, -2, -3).
|
| Growth protocol |
Human aorta VMSCs were cultured in Dulbecco’s modified Eagle’s medium containing 10% FBS at 37°C in a 5% CO2 atmosphere. Medium was renewed every 2 to 3 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent. miRNeasy Mini Kit (Cat # 217004， Qiagen) was used with 2 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to Rnor6.0
Supplementary_files_format_and_content: Matrix table with raw gene counts, TPM for every gene and every sample
|
| |
|
| Submission date |
May 14, 2021 |
| Last update date |
May 15, 2021 |
| Contact name |
Li Zhang |
| E-mail(s) |
zhangli4029@126.com
|
| Organization name |
The First Affiliated Hospital of Guangdong Pharmaceutical University
|
| Street address |
No. 19, Nonglinxia Road
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510080 |
| Country |
China |
| |
|
| Platform ID |
GPL24676 |
| Series (1) |
| GSE174451 |
Anti-aging effect of olmesartan on miRNA expression profile in smooth muscle cells |
|
| Relations |
| BioSample |
SAMN19193847 |
| SRA |
SRX10881795 |
