|
| Status |
Public on Apr 16, 2021 |
| Title |
HEK293T-hACE2_cov rep2 |
| Sample type |
SRA |
| |
|
| Source name |
HEK293T-hACE2 cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEK293T
genotype: stable hACE2 expression
treatment: SARS-CoV-2
|
| Treatment protocol |
MOI=1, infect for 72 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extractd total RNA
KAPA RNA HyperPrep with RiboErase
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Basecalls performed using CASAVA version 1.8
Subread 2.01 was used for alignment
featureCount was used to extract read count
Genome_build: hg38
Supplementary_files_format_and_content: gene count txt fule
|
| |
|
| Submission date |
Mar 18, 2021 |
| Last update date |
Apr 16, 2021 |
| Contact name |
Guihua Sun |
| E-mail(s) |
gusun@coh.org
|
| Phone |
6262182019
|
| Organization name |
City of hope
|
| Department |
Department of Neurodegenerative Diseases
|
| Street address |
1500 East Duarte Rd.
|
| City |
Duarte |
| State/province |
CA |
| ZIP/Postal code |
91010 |
| Country |
USA |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE169158 |
Host transcriptome response to SARS-CoV2 infection |
|
| Relations |
| BioSample |
SAMN18350151 |
| SRA |
SRX10375212 |
