|
Status |
Public on Apr 16, 2021 |
Title |
HEK293T-hACE2_cov rep2 |
Sample type |
SRA |
|
|
Source name |
HEK293T-hACE2 cell line
|
Organism |
Homo sapiens |
Characteristics |
cell line: HEK293T genotype: stable hACE2 expression treatment: SARS-CoV-2
|
Treatment protocol |
MOI=1, infect for 72 hours
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extractd total RNA KAPA RNA HyperPrep with RiboErase
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Data processing |
Basecalls performed using CASAVA version 1.8 Subread 2.01 was used for alignment featureCount was used to extract read count Genome_build: hg38 Supplementary_files_format_and_content: gene count txt fule
|
|
|
Submission date |
Mar 18, 2021 |
Last update date |
Apr 16, 2021 |
Contact name |
Guihua Sun |
E-mail(s) |
gusun@coh.org
|
Phone |
6262182019
|
Organization name |
City of hope
|
Department |
Department of Neurodegenerative Diseases
|
Street address |
1500 East Duarte Rd.
|
City |
Duarte |
State/province |
CA |
ZIP/Postal code |
91010 |
Country |
USA |
|
|
Platform ID |
GPL16791 |
Series (1) |
GSE169158 |
Host transcriptome response to SARS-CoV2 infection |
|
Relations |
BioSample |
SAMN18350151 |
SRA |
SRX10375212 |