|
| Status |
Public on Mar 17, 2021 |
| Title |
AC-2 |
| Sample type |
SRA |
| |
|
| Source name |
tumor sample
|
| Organism |
Mus musculus |
| Characteristics |
strain: BALB/c
treatment: AC treated
tissue: colon tumor
|
| Treatment protocol |
Tumor induction:CT26 cells were injected into syngeneic BALB/c mice
Sample treatment: model was given saline once a day (6 g / kg) and 'AC' treatment was given Astragalus and Curcuma phaeocaulis Valeton(2:1) once a day (6 g / kg).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent. VAHTS® Universal V6 RNA-seq Library Prep Kit for Illumina was used with 5 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
FeatureCounts v1.5.0-p3 was used to count the reads numbers mapped to each gene. And then FPKM of each gene was calculated based on the length of the gene and reads count mapped to this gene. FPKM, expected number of Fragments Per Kilobase of transcript sequence per Millions base pairs sequenced, considers the effect of sequencing depth and gene length for the reads count at the same time, and is currently the most commonly used method for estimating gene expression levels.
Genome_build: mm9
Supplementary_files_format_and_content: tab-delimited text files include raw counts and FPKM values for each Sample
|
| |
|
| Submission date |
Mar 16, 2021 |
| Last update date |
Mar 17, 2021 |
| Contact name |
Fei jun Gu |
| E-mail(s) |
Fery0123@126.com
|
| Phone |
15895841636
|
| Organization name |
Nanjing University of traditional Chinese Medicine
|
| Street address |
138 Xianlin Avenue
|
| City |
Nanjing |
| ZIP/Postal code |
210046 |
| Country |
China |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE169005 |
Transcriptomics analysis of mouse tumor sample |
|
| Relations |
| BioSample |
SAMN18320150 |
| SRA |
SRX10355026 |
