|
| Status |
Public on Oct 05, 2021 |
| Title |
ASZ_RasTGFbKD_Fos_rep2 |
| Sample type |
SRA |
| |
|
| Source name |
ASZ001 murine BCC cell line
|
| Organism |
Mus musculus |
| Characteristics |
cell line: ASZ001
genotype: RasV12_TGFbR1kd
treatment: Dox-4OHT
chip antibody: Rabbit anti-c-FOS mAb (9F6) (CST)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Six days after Dox + 4OHT induction, ASZ_RasV12_TGFβR1kd cells were crosslinked and chromatin immunoprecipitation for c-FOS was carried out as described in the methods section of the manuscript.
ChIP libraries were prepared for sequencing using standard Illumina protocols.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
bowtie2 used for alignment.
Duplicate fragments were removed using samtools1.8.
macs2 used for peak calling.
IDR analysis for generating reproducible peaks.
Genome_build: mm9
Supplementary_files_format_and_content: bed
|
| |
|
| Submission date |
Mar 05, 2021 |
| Last update date |
Oct 05, 2021 |
| Contact name |
Anthony Oro |
| Organization name |
Stanford University
|
| Department |
Dermatology
|
| Street address |
269 Campus Drvie
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (2) |
| GSE168374 |
DNA binding profile of c-FOS in experimentally-induced basal to squamous cell carcinoma transition (BST) |
| GSE168376 |
C-FOS-driven basal to squamous cell transition |
|
| Relations |
| BioSample |
SAMN18170499 |
| SRA |
SRX10247303 |
