|
| Status |
Public on Feb 05, 2021 |
| Title |
Ebf2-EGFP E15pos cells scRNA-seq rep1 |
| Sample type |
SRA |
| |
|
| Source name |
cerebral cortex
|
| Organism |
Mus musculus |
| Characteristics |
cell type: cortical Ebf2-EGFP+ cells
strain: Tg(Ebf2-EGFP)FB58Gsat
tissue: cerebral cortex
developmental stage: E15.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ebf2-EGFP positive cells at E15.5 were dissociated, resuspended and purified by FACS, about eight embryos from two timed-pregnant mice were used in the experiments.
Approximately 3000 cells were processed for downstream reverse transcription, cDNA synthesis and library preparation according to 10× Genomics Chromium Single Cell protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
single cell RNA
|
| Data processing |
Samples were sequenced on Illumina HiSeq2500 platform using 150 base paired-end reads. Sequencing reads were analyzed by Cell Ranger (v2.0.0).
The gene expression datasets were analyzed by Seurat (v2.0) and Monocle package.
Genome_build: mm10
Supplementary_files_format_and_content: csv files include FPKM values for each Sample ...
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample
|
| |
|
| Submission date |
Feb 02, 2021 |
| Last update date |
Feb 05, 2021 |
| Contact name |
Jia Li |
| E-mail(s) |
jli108@bwh.harvard.edu
|
| Organization name |
Tongji University
|
| Street address |
No.67 chifeng road, yangpu district
|
| City |
Shanghai |
| ZIP/Postal code |
2000665 |
| Country |
China |
| |
|
| Platform ID |
GPL17021 |
| Series (2) |
| GSE166014 |
Integrative genomic analysis of early neurogenesis reveals a temporal genetic program for differentiation and specification of preplate and Cajal-Retzius neurons [scRNA-Seq] |
| GSE166015 |
Integrative genomic analysis of early neurogenesis reveals a temporal genetic program for differentiation and specification of preplate and Cajal-Retzius neurons |
|
| Relations |
| BioSample |
SAMN17763639 |
| SRA |
SRX10006793 |
