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| Status |
Public on Feb 05, 2021 |
| Title |
Integrative genomic analysis of early neurogenesis reveals a temporal genetic program for differentiation and specification of preplate and Cajal-Retzius neurons [scRNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Neurogenesis in the developing neocortex begins with the generation of the preplate, which consists of early-born neurons including Cajal-Retzius (CR) cells and subplate neurons. Here, utilizing the Ebf2-EGFP transgenic mouse in which EGFP initially labels the preplate neurons then persists in CR cells, we reveal the dynamic transcriptome profiles of early neurogenesis and CR cell differentiation. Genome-wide RNA-seq and ChIP-seq analyses at multiple early neurogenic stages have revealed the temporal gene expression dynamics of early neurogenesis and distinct histone modification patterns in early differentiating neurons. We have identified a new set of coding genes and lncRNAs involved in early neuronal differentiation and validated with functional assays in vitro and in vivo. In addition, at E15.5 when Ebf2-EGFP+ cells are mostly CR neurons, single-cell sequencing analysis of purified Ebf2-EGFP+ cells uncovers molecular heterogeneity in CR neurons, but without apparent clustering of cells with distinct regional origins. Along a pseudotemporal trajectory these cells are classified into three different developing states, revealing genetic cascades from early generic neuronal differentiation to late fate specification during the establishment of CR neuron identity and function. Our findings shed light on the molecular mechanisms governing the early differentiation steps during cortical development, especially CR neuron differentiation.
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| |
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| Overall design |
single cell mRNA profile of cortical Ebf2-EGFP+ cells at mouse embryonic stage E15.5
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| |
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| Contributor(s) |
Li J, Sun L, Shen Q |
| Citation(s) |
33760820 |
| |
| Submission date |
Feb 02, 2021 |
| Last update date |
Jun 06, 2021 |
| Contact name |
Jia Li |
| E-mail(s) |
jli108@bwh.harvard.edu
|
| Organization name |
Tongji University
|
| Street address |
No.67 chifeng road, yangpu district
|
| City |
Shanghai |
| ZIP/Postal code |
2000665 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE166015 |
Integrative genomic analysis of early neurogenesis reveals a temporal genetic program for differentiation and specification of preplate and Cajal-Retzius neurons |
|
| Relations |
| BioProject |
PRJNA698981 |
| SRA |
SRP304344 |
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