|
| Status |
Public on Jul 31, 2011 |
| Title |
VIHCC P_M461 |
| Sample type |
RNA |
| |
|
| Source name |
Human hepatocellular carcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: hepatocellular carcinoma
vascular invasion: No
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Using formalin-fixed, paraffin-embedded HCC tissues, total RNA was extracted using TRIzol (Invitrogen).
|
| Label |
Cy5
|
| Label protocol |
Refer to the Illumina beadarray Assay System Manual
|
| |
|
| Hybridization protocol |
Refer to the Illumina beadarray Assay System Manual
|
| Scan protocol |
Refer to the Illumina beadarray Assay System Manual
|
| Description |
none
|
| Data processing |
For each probe, replicate mesurements were averaged after removing outliers. After subtracting background signal intensities and applying a floor value of 0.01 was assigned to negative values, data were normalized using the cubic spline algorithm implemented in GenePattern IlluminaNormalizer module.
|
| |
|
| Submission date |
Jan 22, 2010 |
| Last update date |
Jul 31, 2011 |
| Contact name |
Yujin Hoshida |
| E-mail(s) |
Yujin.Hoshida@UTSouthwestern.edu
|
| Organization name |
University of Texas Southwestern Medical Center
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL8432 |
| Series (2) |
| GSE20017 |
Gene Signature to Identify Vascular Invasion in Hepatocellular Carcinoma |
| GSE20238 |
Gene Signature to Identify Vascular Invasion in Human Hepatocellular Carcinoma |
|
| Relations |
| Reanalysis of |
GSM499216 |
