|
| Status |
Public on Jan 27, 2021 |
| Title |
severely contused muscel at 3h , biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
skeletal muscle from wound site, severely contused, 3h
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: the thigh muscles of the right posterior limb
treatment: 3h after severe contusion
age: 10-12 weeks
|
| Treatment protocol |
(1) Control group (n = 3): no treatment before sampling; (2) Mild contusion group (n = 15): a 500-g counterpoise weight (diameter, 0.8 cm) fell from a height of 15 cm through a clear Lucite tube onto the thigh muscles of the right posterior limb, with an energy of ~0.74 J (calculated by E = mgh) transferred to the limb; (3) Severe contusion group (n = 15): a 500-g counterpoise weight (diameter, 1.1 cm ) fell from a height of 50 cm through a clear Lucite tube onto the thigh muscles of the right posterior limb, with an energy of ~2.45 J transferred to the limb (n = 15).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the skeletal muscle specimens
|
| Label |
biotin
|
| Label protocol |
The Ambion WT Expression Kit generates purified sense-strand cDNA (with incorporated dUTP) that is ready for fragmentation and labeling using the Affymetrix GeneChip WT Terminal Labeling Kit.
|
| |
|
| Hybridization protocol |
Hybridization for array plates (Gene 2.0 ST Arrays;Affymetrix®) was used the GeneChip® Hybridization, Wash and Stain Kit according to the manufacturer’s protocol.
|
| Scan protocol |
GeneChips were scanned by GeneChip Scanner 3000 7G
|
| Description |
Gene expression at 3h after severe contusion
|
| Data processing |
The data were analyzed with Transcriptome Analysis Console software (version 4.0.1; Affymetrix®)
|
| |
|
| Submission date |
Dec 02, 2020 |
| Last update date |
Jan 27, 2021 |
| Contact name |
Jun-hong Sun |
| E-mail(s) |
junhong.sun@sxmu.edu.cn
|
| Organization name |
Shanxi Medical University
|
| Department |
School of Forensic Medicine
|
| Street address |
98 University Street, Yuci District, Jinzhong
|
| City |
Jinzhong |
| State/province |
Shanxi Province |
| ZIP/Postal code |
030604 |
| Country |
China |
| |
|
| Platform ID |
GPL17117 |
| Series (1) |
| GSE162565 |
Gene expression Profiles following mild and severe muslce contusion in rats |
|
