|
| Status |
Public on Oct 26, 2020 |
| Title |
E15.5 (2) |
| Sample type |
RNA |
| |
|
| Source name |
Sorted Cxcl12-DsRed+ stromal cells from E15.5 livers
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
genotype/variation: Cxcl12DsRed/+
developmental stage: E15.5
tissue: liver
cell type: CD45-Ter119-Cxcl12-DsRed+ stromal cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
The labelled cDNA was hybridized to Affymetrix Mouse Gene ST 1.0 chips following the manufacturer's instructions.
|
| Scan protocol |
standard Affymetrix protocol
|
| Data processing |
Expression values for all probes were normalized and determined using the robust multi-array average (RMA) method with an Affymetrix Expression Console (Irizarry et al., 2003). Normalized data were analyzed using Transcriptome Analysis Console 2.0.
|
| |
|
| Submission date |
Oct 09, 2020 |
| Last update date |
Oct 27, 2020 |
| Contact name |
Yeojin Lee |
| E-mail(s) |
yl3155@cumc.columbia.edu
|
| Organization name |
Columbia University Medical Center
|
| Lab |
Lei Ding
|
| Street address |
650 WEST 168TH STREET, BB 1108
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE159308 |
Gene expression profile of Cxcl12-DsRed+ stromal cells from E15.5, P5, and P10 livers. |
|
