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| Status |
Public on Oct 26, 2020 |
| Title |
Gene expression profile of Cxcl12-DsRed+ stromal cells from E15.5, P5, and P10 livers. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The study is designed to identify transcriptional differences of CD45-Ter119-Cxcl12-DsRed+ stromal cells at different develomental stages. 50,000 CD45-Ter119-Cxcl12-DsRed+ stromal cells from freshly prepared livers of E15.5, P5 and P10 Cxcl12DsRed/+ mice were sorted into Trizol by flow cytometry. Total RNA was isolated and amplified with the WT-Ovation Pico RNA Amplification system (Nugen) as the manufacturer’s instructions. Sense strand complementary DNA was generated using the WT-Ovation Exon Module (Nugen). Then, cDNA was fragmented and labelled using FL-Ovation DNA Biotin Module V2 (Nugen). The labelled cDNA was hybridized to Affymetrix Mouse Gene ST 1.0 chips following the manufacturer's instructions.
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| Overall design |
Three independent replicates for each conditions.
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| |
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| Contributor(s) |
Ding L, Lee Y |
| Citation(s) |
33151261 |
| |
| Submission date |
Oct 09, 2020 |
| Last update date |
Jan 26, 2021 |
| Contact name |
Yeojin Lee |
| E-mail(s) |
yl3155@cumc.columbia.edu
|
| Organization name |
Columbia University Medical Center
|
| Lab |
Lei Ding
|
| Street address |
650 WEST 168TH STREET, BB 1108
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA668339 |
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