|
| Status |
Public on Jun 14, 2023 |
| Title |
NL 6hpi [B2_CRRA200000231-1a_HT7GVDSXX_L3_1] |
| Sample type |
SRA |
| |
|
| Source name |
corneas
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
disease: Normal
tissue: Corneas
treatment: infection with 10000 CFU of PA
time point: 6h
|
| Treatment protocol |
Corneas from normal and diabetic mice were gently scratched with 26‐gauge needles, followed by topical inoculation of Pseudomonas Aeruginosa (PA).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Corneas were collected, and total RNA was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol.
Performed by NOVAGene NGS services.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
B2_CRRA200000231-1a_HT7GVDSXX_L3_1
|
| Data processing |
Star
Data QC
Quantification
DeSeq2
Cluster analysis
Genome_build: mm10 (GRCm38)
Supplementary_files_format_and_content: Partek_quantification_annotation.tar: Tar archive includes raw counts and annotation.
Supplementary_files_format_and_content: DeSeq2-Rs-7-7-2020.txt: DESeq2 results
|
| |
|
| Submission date |
Jul 15, 2020 |
| Last update date |
Jun 14, 2023 |
| Contact name |
WeiPing Chen |
| E-mail(s) |
weipingChen@niddk.nih.gov
|
| Phone |
301-496-0175
|
| Organization name |
NIDDK/NIH
|
| Department |
GCL
|
| Lab |
Genomics Core Lab
|
| Street address |
Bldg 8, Room 1A11, NIDDK/NIH
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE154507 |
Immune response to pathogens in diabetes |
|
| Relations |
| BioSample |
SAMN15545038 |
| SRA |
SRX8738519 |
