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Series GSE154507 Query DataSets for GSE154507
Status Public on Jun 14, 2023
Title Immune response to pathogens in diabetes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To investigate the differential gene expression by NGS technology in normal and diabetic corneas during Pseudomonas Aeruginosa (PA) infection, corneas from normal and diabetic mouse were gently scratched with 26‐gauge needles, followed by topical inoculation of PA. At 6 h, 1 and 3 days, the corneas were collected and processed for RNA sequencing. Bioinformatic analyses revealed an orderly activation of biological processes starting from pathogen pattern recognition, cytokine/chemokine expression, to innate immune cell infiltration (chemotaxis), and to the activation of TH2 and TH17 immunity in NL corneas. Diabetes disturbs these orderly innate and adaptive immune responses, leading to increased susceptibility and exacerbated progression of microbial keratitis. Completion of the proposed study should reveal novel insights into the molecular and cellular aspects of the proneness of diabetic patients to infection and may enable the development of new therapeutic modalities for enhancing mucosal innate immunity in diabetic patients, and for treating infectious keratitis in general.
 
Overall design Examination of differential gene expression in normal and diabetic mouse corneas.
 
Contributor(s) Yu F
Citation(s) 37279395
Submission date Jul 15, 2020
Last update date Jun 14, 2023
Contact name WeiPing Chen
E-mail(s) weipingChen@niddk.nih.gov
Phone 301-496-0175
Organization name NIDDK/NIH
Department GCL
Lab Genomics Core Lab
Street address Bldg 8, Room 1A11, NIDDK/NIH
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (27)
GSM4672312 NL [A1_CRRA200000224-1a_HT7GVDSXX_L3_1]
GSM4672313 NL [A2_CRRA200000225-1a_HT7GVDSXX_L3_1]
GSM4672314 NL [A3_CRRA200000226-1a_HT7GVDSXX_L3_1]
Relations
BioProject PRJNA646505
SRA SRP272121

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE154507_DeSeq2-Rs-7-7-2020.txt.gz 9.7 Mb (ftp)(http) TXT
GSE154507_Partek_quantification_annotation.tar.gz 2.3 Mb (ftp)(http) TAR
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Raw data are available in SRA
Processed data are available on Series record
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