|
| Status |
Public on Jan 01, 2021 |
| Title |
SCAP at c, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
dental (apical papilla)
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: control
|
| Treatment protocol |
For osteogenic differentiation, when cells reached 80% confluence, the medium was changed to osteogenic medium containing 10 mM β-glycerolphosphate (Sigma-Aldrich, St. Louis, MO, USA), 10 nM dexamethasone (Sigma-Aldrich) and 50 μg/mL L-ascorbic acid (Sigma-Aldrich). The osteogenic medium was changed every 3 days.
|
| Growth protocol |
Then cells were cultured in α-minimum essential medium (HyClone, Logan, UT, United States) supplemented with 20% fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA), 100 U/ml penicillin and 100 μg/ml streptomycin (HyClone) at 37°C in a 5% CO2 incubator. The medium was changed every 3 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted by mirVanaTM RNA Isolation Kit (AM1561 ) following the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared from 0.2 μg RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 48C on GeneChip miRNA Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneArray Scanner 3000.
|
| Description |
Gene expression data from SCAP without differentiation.
|
| Data processing |
The data were analyzed with Expression Console ™ Software 1.3 using Affymetrix default analysis settings and global scaling as normalization method.The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Jul 15, 2020 |
| Last update date |
Jan 03, 2021 |
| Contact name |
Junqing Liu |
| Organization name |
Shandong University
|
| Street address |
No.44-1 Wenhua Road West
|
| City |
Jinan |
| ZIP/Postal code |
250012 |
| Country |
China |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE154466 |
Expression data of microRNA from human stem cells from apical papilla |
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