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Series GSE154466 Query DataSets for GSE154466
Status Public on Jan 01, 2021
Title Expression data of microRNA from human stem cells from apical papilla
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Expression profiling by array
Summary Osteo/odontogenic differentiation is a key process of human stem cells from apical papilla (SCAP) in tooth root development. Emerging evidence indicates microRNAs (miRNAs) play diverse roles in osteogenesis. However, their functions in osteo/odontogenic differentiation of SCAP remain to be elucidated.
We used miRNA microarray to analysis differentially expressed miRNAs in order to investigate the role of miRNAs in SCAP osteo/odontogenic differentiation and underlying mechanism.
Overall design Stem cells from apical papilla of human immature third molar were selected for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain homogeneous stem cells from apical papilla (SCAP) at each osteogenic differentiation stage in order to increase the temporal resolution of expression profiles. To that end, we selected SCAP at two time-points: before osteogenic differentiation (c) and osteogenic differentiation for 7 days (k).Biological replicates: 3 control replicates (1c, 2c, 3c), 3 testing replicates (1k, 2k, 3k).
Contributor(s) Wang Y, Liu J, Du J
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Submission date Jul 15, 2020
Last update date Jan 04, 2021
Contact name Junqing Liu
Organization name Shandong University
Street address No.44-1 Wenhua Road West
City Jinan
ZIP/Postal code 250012
Country China
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (6)
GSM4671480 SCAP at c, biological rep1
GSM4671481 SCAP at c, biological rep2
GSM4671482 SCAP at c, biological rep3
BioProject PRJNA646393

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE154466_RAW.tar 4.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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