|
| Status |
Public on Oct 31, 2009 |
| Title |
donor 3, H5N1 infected macrophages at 3h |
| Sample type |
RNA |
| |
|
| Source name |
macrophages from donor 3 infected with H5N1 at 3h post-infection time
|
| Organism |
Homo sapiens |
| Characteristics |
individual: donor 3
infection: H5N1
time point: 3h post-infection time
cell type: monocyte-derived macrophages
|
| Treatment protocol |
Differentiated macrophages were infected with H1N1, H5N1 or mock infection at a multiplicity of infection (MOI) of two. Total RNA was extracted from cells after 1, 3, and 6h post-infection
|
| Growth protocol |
Peripheral-blood leucocytes were separated from buffy coats of healthy donors by Ficoll-Paque density gradient centrifugation and purified by adherence. The cells were allowed to differentaite for 14 days in RPMI 1640 supplemented with 5% heat-inactivated autologous plasma.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by RNEasy Mini kit (Qiagne) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNA was labeled employing the Whole Transcript (WT) Sense Target Labeling Assay according to the manufacturer’s instructions (Affymetrix).
|
| |
|
| Hybridization protocol |
The samples were hybridized to Human Gene 1.0 ST microarrays according to standardized protocols from Affymetrix.
|
| Scan protocol |
The samples were scanned on a GeneChip Scanner 3000 7G (Affymetrix).
|
| Description |
Gene expression data of influenza A H5N1 infected macrophages at 3 h post-infection time
|
| Data processing |
The microarray data were preprocessed with Affymetrix Expression Console software using Affymetrix default Robust Multichip Analysis (RMA) sketch algorithm workflow.
|
| |
|
| Submission date |
Oct 30, 2009 |
| Last update date |
Sep 01, 2016 |
| Contact name |
SMY Lee |
| Organization name |
The University of Hong Kong
|
| Street address |
21 Sassoon Road
|
| City |
Hong Kong |
| ZIP/Postal code |
HKG |
| Country |
Hong Kong |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE18816 |
Expression data of influenza A infected human macrophages |
|
| Relations |
| Reanalyzed by |
GSE86357 |
