|
| Status |
Public on Jan 19, 2010 |
| Title |
muscle_diabetic_16413 |
| Sample type |
RNA |
| |
|
| Source name |
Skeletal muscle, vastus lateralis
|
| Organism |
Homo sapiens |
| Characteristics |
t2dmknown: 1
dmdebut: 2002
diabetesdisposition: 0
euglycemia: 0
igt: 0
dmverified: 1
glycemiagroup: 3
obesity: 0
hypertension: 1
dyslipidemia: 1
metabolic_syndrome: 1
age: 58
bmi: 25.36
whr: 0.96
vo2: 1.75
vo2_kg: 20.81
log_vo2_kg: 1.318
vo2_kg_ffm: 32.5
log_vo2_kg_ffm: 1.512
dietonly: 0
sulfonyl: 0
metformin: 1
tzd: 0
statin: 0
fibrat: 0
asa: 0
ltpa_score: 3
smokercurrent: 0
smokerex: 1
smokerstopyear: 2002
examination: 17/09/2004
born: 1946-02-05
gender: 1
height: 182
weight: 84
hip: 100
waist: 96
bpsyst: 160
bpdiast: 95
heartrate: 77
bpancle: 160
b_hgb: 9.6
evf: 0.46
baso: 0.03
log_baso: -1.523
eosino: 0.15
log_eosino: -0.824
lymfo: 2.2
log_lymfo: 0.342
mono: 0.37
log_mono: -0.432
neutro: 3.6
log_neutro: 0.556
leuko: 6.3
fibrinogen: 9
albumin_0h: 44.2
albumin_1h: 46.9
albumin_2h: 39.9
crp: 6
log_crp: 0.778
orosomucoid: 0.79
log_orosomucoid: -0.102
myoglobin: 55
basp: 56
ck: 102
ldh: 180
asat: 32
bilirubiner: 27
cholesteroler: 4.9
hdl: 1.2
log_hdl: 0.079
ldl: 3.3
tag: 1.22
log_tag: 0.086
creatininium: 0.094
natrium: 140
kalium: 3.7
calcium: 2.36
glucose_0h: 6.7
log_glucose_0h: 0.826
glucose_1h: 16.6
log_glucose_1h: 1.22
glucose_2h: 18.4
log_glucose_2h: 1.265
tsh: 6.1
insulin_0h: 38
log_insulin_0h: 1.58
insulin_1h: 202
log_insulin_1h: 2.305
insulin_2h: 226
log_insulin_2h: 2.354
homa1_ir: 1.886
log_homa1_ir: 0.276
homa2_ir: 0.77
log_homa2_ir: -0.116
hba1c: 6.7
log_hba1c: 0.826
dxa_hardware: 2
arms_bmd: 0.5
arms_fm: 2.1
arms_lm: 6.9
arms_sum: 9.4
legs_bmd: 1.3
legs_fm: 6.1
legs_lm: 17.3
legs_sum: 24.6
limb_bmd: 1.7
limb_fm: 8.1
limb_lm: 24.2
limb_sum: 34.1
trunk_bmd: 1.2
trunk_fm: 15.4
trunk_lm: 25.5
trunk_sum: 42.1
total_bmd: 3.4
total_fm: 24.4
total_lm: 53.8
total_sum: 81.6
trunk_limb_fm_ratio: 1.9
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Muscle was homogenized in Trizol with Polytron motor driven homogenizer and RNA extraction carried out using Trizol protocol (Invitrogen). Hybridization, washing, staining and scanning of the arrays were performed according to manufacturer's instructions (Affymetrix).
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
Total RNA was prepared for hybridization using the protocol published by Affymetix
|
| Data processing |
All array data were normalized using RMA and the Microarray Suite version 5.0 (MAS 5.0) algorithm to a global scaling intensity of 100. Data were filtered using present/absent calls. Probes called absent by MAS 5.0 across all arrays were discarded.
|
| |
|
| Submission date |
Oct 26, 2009 |
| Last update date |
Jul 23, 2012 |
| Contact name |
Iain Gallagher |
| E-mail(s) |
iaingallagher@gmail.com
|
| Phone |
00 44 1786 46 6024
|
| Organization name |
University of Stirling
|
| Department |
Faculty of Health Sciences and Sport
|
| Street address |
Room 4B133 Cottrell Building, University of Stirling, Airthrey Road
|
| City |
Stirling |
| ZIP/Postal code |
FK9 4LA |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL9486 |
| Series (1) |
| GSE18732 |
mRNA expression data from skeletal muscle of type 2 diabetes |
|
