|
| Status |
Public on Jan 19, 2010 |
| Title |
muscle_normal_16149 |
| Sample type |
RNA |
| |
|
| Source name |
Skeletal muscle, vastus lateralis
|
| Organism |
Homo sapiens |
| Characteristics |
t2dmknown: 0
dmdebut: 0
diabetesdisposition: 0
euglycemia: 1
igt: 0
dmverified: 0
glycemiagroup: 1
obesity: 0
hypertension: 1
dyslipidemia: 1
metabolic_syndrome: 0
age: 62
bmi: 29.39
whr: 1.14
vo2: 1.66
vo2_kg: 23.57
log_vo2_kg: 1.372
vo2_kg_ffm: 48.9
log_vo2_kg_ffm: 1.69
dietonly: 0
sulfonyl: 0
metformin: 0
tzd: 0
statin: 0
fibrat: 0
asa: 0
ltpa_score: 1
smokercurrent: 0
smokerex: 0
smokerstopyear: 0
examination: 2005-11-01
born: 1942-08-04
gender: 0
height: 155
weight: 70.6
hip: 92.5
waist: 105
bpsyst: 150
bpdiast: 80
heartrate: 66
bpancle: 150
b_hgb: 8
evf: 0.41
baso: 0.07
log_baso: -1.155
eosino: 0.29
log_eosino: -0.538
lymfo: 2.5
log_lymfo: 0.398
mono: 0.51
log_mono: -0.292
neutro: 3.6
log_neutro: 0.556
leuko: 7
fibrinogen: 9
albumin_0h: 45.3
albumin_1h: 44.7
albumin_2h: 43.9
crp: 3
log_crp: 0.477
orosomucoid: 0
log_orosomucoid: 0
myoglobin: 34
basp: 55
ck: 51
ldh: 170
asat: 24
bilirubiner: 7
cholesteroler: 5.3
hdl: 2.2
log_hdl: 0.342
ldl: 2.6
tag: 0.99
log_tag: -0.004
creatininium: 0.056
natrium: 142
kalium: 4.2
calcium: 2.36
glucose_0h: 4.4
log_glucose_0h: 0.643
glucose_1h: 9
log_glucose_1h: 0.954
glucose_2h: 6.2
log_glucose_2h: 0.792
tsh: 3.46
insulin_0h: 37
log_insulin_0h: 1.568
insulin_1h: 341
log_insulin_1h: 2.533
insulin_2h: 332
log_insulin_2h: 2.521
homa1_ir: 1.206
log_homa1_ir: 0.081
homa2_ir: 0.67
log_homa2_ir: -0.173
hba1c: 5.6
log_hba1c: 0.748
dxa_hardware: 2
arms_bmd: 0.3
arms_fm: 3.9
arms_lm: 4.1
arms_sum: 8.2
legs_bmd: 0.7
legs_fm: 9.9
legs_lm: 10.3
legs_sum: 20.9
limb_bmd: 1
limb_fm: 13.8
limb_lm: 14.4
limb_sum: 29.1
trunk_bmd: 0.7
trunk_fm: 18.8
trunk_lm: 16.8
trunk_sum: 36.3
total_bmd: 2.2
total_fm: 33.5
total_lm: 34
total_sum: 69.7
trunk_limb_fm_ratio: 1.36
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Muscle was homogenized in Trizol with Polytron motor driven homogenizer and RNA extraction carried out using Trizol protocol (Invitrogen). Hybridization, washing, staining and scanning of the arrays were performed according to manufacturer's instructions (Affymetrix).
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
Total RNA was prepared for hybridization using the protocol published by Affymetix
|
| Data processing |
All array data were normalized using RMA and the Microarray Suite version 5.0 (MAS 5.0) algorithm to a global scaling intensity of 100. Data were filtered using present/absent calls. Probes called absent by MAS 5.0 across all arrays were discarded.
|
| |
|
| Submission date |
Oct 26, 2009 |
| Last update date |
Jul 23, 2012 |
| Contact name |
Iain Gallagher |
| E-mail(s) |
iaingallagher@gmail.com
|
| Phone |
00 44 1786 46 6024
|
| Organization name |
University of Stirling
|
| Department |
Faculty of Health Sciences and Sport
|
| Street address |
Room 4B133 Cottrell Building, University of Stirling, Airthrey Road
|
| City |
Stirling |
| ZIP/Postal code |
FK9 4LA |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL9486 |
| Series (1) |
| GSE18732 |
mRNA expression data from skeletal muscle of type 2 diabetes |
|
