|
| Status |
Public on Mar 31, 2021 |
| Title |
O6: p35S:AtBBD1 seedlings treated with 20% PEG for 6 hours |
| Sample type |
SRA |
| |
|
| Source name |
Leaves
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: Leaves
age: 3-weeks-old
treatment: Stress with 20% PEG for 6 hours
|
| Treatment protocol |
Plants were watered with 20% PEG to induce drought condition.
|
| Growth protocol |
Plant were growed at 23°C long day condition on soil.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using trizol reagent
mRNA libraries were prepared 1 μg of total RNA using TruSeq standard mRNA kit (Illumina), according to the manufacturer's instruction.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Illumina Casava1.8.2 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and differentially expressed genes were identified using TopHat2-Cufflinks pipeline
Genome_build: TAIR 10
Supplementary_files_format_and_content: FPKM values for each sample, in excel file
|
| |
|
| Submission date |
Jun 10, 2020 |
| Last update date |
Mar 31, 2021 |
| Contact name |
Zeeshan Nasim |
| E-mail(s) |
zeeshan_ibge@yahoo.com
|
| Organization name |
Korea University
|
| Department |
School of Life Sciences and Biotechnology
|
| Street address |
Anam-ro 145, Seongbuk-Gu
|
| City |
Seoul |
| ZIP/Postal code |
136-713 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL19580 |
| Series (1) |
| GSE152163 |
Transcriptome analysis of p35S:AtBBD1 and atbbd1 (Knock-out) under PEG-induced drought stress condition |
|
| Relations |
| BioSample |
SAMN15196596 |
| SRA |
SRX8516566 |
