|
| Status |
Public on Nov 06, 2020 |
| Title |
BS12 |
| Sample type |
SRA |
| |
|
| Source name |
spleen
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: spleen
age: postnatal day 8
genotype: Clec9a-Cre/Cre Rosa-tdTomato/tdTomato
celltype: TOM+ cDC2
sorting strategy: Live Single Autofluorescence- MHCII+ CD11c+ CD11b+ TOM+
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolation with PicoPure Kit according to protocol. Cells were sorted directly in lysis buffer supplied with the kit
cDNA creation with SMARTseq v4 Kit (Takara), library creation with MicroPlex Library Preparation Kit v2 (Diagenode)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 1500 |
| |
|
| Data processing |
Sequencing reads were aligned to mm10 mouse genome using STAR
Gene expression in transcripts per kilobase million (TPM) was calculated with RSEM
Genome_build: mm10
Supplementary_files_format_and_content: xlsx file contains TPM values for each sample
|
| |
|
| Submission date |
Jun 01, 2020 |
| Last update date |
Nov 07, 2020 |
| Contact name |
Nikolaos Papaioannou |
| E-mail(s) |
Nikolaos.Papaioannou@med.uni-muenchen.de
|
| Organization name |
Biomedical Center
|
| Street address |
Großhaderner Straße 9
|
| City |
Planegg-Martinsried |
| ZIP/Postal code |
82152 |
| Country |
Germany |
| |
|
| Platform ID |
GPL18480 |
| Series (2) |
| GSE151593 |
RNAseq of splenic dendritic cells from 1 week-old and adult Clec9a-Cre Rosa-Tomato mice |
| GSE151595 |
Environmental signals rather than layered ontogeny imprint the function of type 2 conventional dendritic cells in young and adult mice |
|
| Relations |
| BioSample |
SAMN15077615 |
| SRA |
SRX8451731 |
