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Sample GSM4586393 Query DataSets for GSM4586393
Status Public on Nov 06, 2020
Title BS12
Sample type SRA
Source name spleen
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: spleen
age: postnatal day 8
genotype: Clec9a-Cre/Cre Rosa-tdTomato/tdTomato
celltype: TOM+ cDC2
sorting strategy: Live Single Autofluorescence- MHCII+ CD11c+ CD11b+ TOM+
Extracted molecule total RNA
Extraction protocol RNA isolation with PicoPure Kit according to protocol. Cells were sorted directly in lysis buffer supplied with the kit
cDNA creation with SMARTseq v4 Kit (Takara), library creation with MicroPlex Library Preparation Kit v2 (Diagenode)
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1500
Data processing Sequencing reads were aligned to mm10 mouse genome using STAR
Gene expression in transcripts per kilobase million (TPM) was calculated with RSEM
Genome_build: mm10
Supplementary_files_format_and_content: xlsx file contains TPM values for each sample
Submission date Jun 01, 2020
Last update date Nov 07, 2020
Contact name Nikolaos Papaioannou
Organization name Biomedical Center
Street address Großhaderner Straße 9
City Planegg-Martinsried
ZIP/Postal code 82152
Country Germany
Platform ID GPL18480
Series (2)
GSE151593 RNAseq of splenic dendritic cells from 1 week-old and adult Clec9a-Cre Rosa-Tomato mice
GSE151595 Environmental signals rather than layered ontogeny imprint the function of type 2 conventional dendritic cells in young and adult mice
BioSample SAMN15077615
SRA SRX8451731

Supplementary file Size Download File type/resource 173.0 Kb (ftp)(http) TAB
GSM4586393_BS12_91_PND8_Pos_rsem.genes.results.txt.gz 446.1 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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