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| Status |
Public on May 22, 2020 |
| Title |
lag3nc_isotype_tx |
| Sample type |
SRA |
| |
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| Source name |
tumor tissue
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| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tumor: MC38
genotype/variation: mutant M38
treatment: isotype
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| Treatment protocol |
Generation of single-cell suspensions from MC38 tumors, fluorescence acivated cell sorting for T cells
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
10x 3' v2 scRNAseq
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
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| Data processing |
Demultiplexing using CellRanger mkfastq
Alignment and generation of gene/barcode matrices using CellRanger count
Genome_build: mm10
Supplementary_files_format_and_content: gzipped files containing gene/barcode matrices
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| Submission date |
May 21, 2020 |
| Last update date |
May 22, 2020 |
| Contact name |
Anthony Richard Cillo |
| E-mail(s) |
arc85@pitt.edu
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| Organization name |
University of Pittsburgh
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| Department |
Immunology
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| Street address |
The Assembly, Room 4070C Bay 10, 5051 Centre Ave
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| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
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| Platform ID |
GPL19057 |
| Series (1) |
| GSE150991 |
Resistance to PD1 blockade in the absence of metalloprotease-mediated LAG3 shedding |
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| Relations |
| BioSample |
SAMN14992555 |
| SRA |
SRX8377130 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM4563425_lag3nc_isotype_tx.tar.gz |
10.7 Mb |
(ftp)(http) |
TAR |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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