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| Status |
Public on May 22, 2020 |
| Title |
Resistance to PD1 blockade in the absence of metalloprotease-mediated LAG3 shedding |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Mechanisms of resistance to cancer immunotherapy remain poorly understood. Lymphocyte Activation Gene-3 (LAG3) signaling is regulated by A Disintegrin And Metalloprotease-10 (ADAM10) and ADAM17-mediated cell surface shedding. We developed and utilized mutant mice expressing a metalloprotease-resistance version of LAG3 that can be selectively expressed in T cell subsets. We sorted live T cells from tumors of mice bearing MC38 tumors following 3 treatments with either anti-PD1 or IgG control, and performed single-cell RNAseq using 10X 3' v2 reagents.
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| |
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| Overall design |
Investigation of transcription profiles of T cells from tumors of wild-type or mutant M38 bearing mice folllowing treatment with anti-PD1 or isotype
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| |
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| Contributor(s) |
Andrews LP, Cillo AR, Vignali DA |
| Citation(s) |
32680952 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| P01 AI108545 |
Synergies among inhibitory receptors in tolerance, cancer & antiviral immunity |
UNIVERSITY OF PITTSBURGH AT PITTSBURGH |
Dario AA Vignali |
| R01 AI144422 |
Structure, Function and Mechanistic Analysis of LAG3 |
UNIVERSITY OF PITTSBURGH AT PITTSBURGH |
Dario AA Vignali |
| R01 EB022433 |
Lymph node-targeted molecular vaccines |
MASSACHUSETTS INSTITUTE OF TECHNOLOGY |
Darrell J Irvine |
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| |
| Submission date |
May 21, 2020 |
| Last update date |
Aug 21, 2020 |
| Contact name |
Anthony Richard Cillo |
| E-mail(s) |
arc85@pitt.edu
|
| Organization name |
University of Pittsburgh
|
| Department |
Immunology
|
| Street address |
The Assembly, Room 4070C Bay 10, 5051 Centre Ave
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (4)
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| Relations |
| BioProject |
PRJNA634347 |
| SRA |
SRP262640 |
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