|
| Status |
Public on Feb 28, 2021 |
| Title |
KPL4 cells, control, biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Inflammatory breast cancer cells, control
|
| Organism |
Homo sapiens |
| Characteristics |
genotype: Control
cell line: KPL4
|
| Treatment protocol |
N/A
|
| Growth protocol |
KPL4 cells were maintained in HAM’s-F12 media supplemented with 7% fetal bovine serum, 10 mM HEPES, 5 μg/mL insulin, 1μg/mL hydrocortisone at 37°C in 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Qiagen RNeasy Mini kit according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
cDNA was prepared according to the standard Affymetrix protocol from 250 ng total RNA (Expression Analysis Technical Manual, Affymetrix)
|
| |
|
| Hybridization protocol |
5.5 μg of fragmented biotinilated cDNA was hybridized for 16 hr at 45C to Clariom D Human Arrays. GeneChips were washed and stained with streptavidin-phycoerythrin
|
| Scan protocol |
GeneChips were scanned using the A GeneChip 3000 7G.
|
| Description |
Gene expression data from control KPL4 Inflammatory breast cancer cells
|
| Data processing |
cel files were normalized with RMA in Transcriptome Analysis Console (TAC) Software using default settings
|
| |
|
| Submission date |
May 04, 2020 |
| Last update date |
Mar 01, 2021 |
| Contact name |
Christoforos Thomas |
| E-mail(s) |
thomaschristoforos@gmail.com
|
| Organization name |
University of Pennsylvania
|
| Street address |
3400 Civic Center Blvd
|
| City |
Philadelphia |
| State/province |
Pennsylvania |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE149852 |
Expression data from Inflammatory breast cancer (IBC) cells |
|
