|
| Status |
Public on Dec 21, 2021 |
| Title |
TB13-3 |
| Sample type |
RNA |
| |
|
| Source name |
AML cell line expressing Trib1 and Bcl11a
|
| Organism |
Mus musculus |
| Characteristics |
cell line: Trib1- and Bcl11a-expressing murine AML cells
|
| Treatment protocol |
No treatment was provided.
|
| Growth protocol |
Trib1, TB13 and TB14 cells were grown in IMDM supplemented with 10% fetal bovine serum, and 5 ng/ml IL-3.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using RNA Easy kit (QIAGEN) according to the manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 7.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 PM Array. GeneChips were washed and stained in the Affymetrix GeneAtlas Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneAtlas Imaging Station.
|
| Description |
Gene expression data from mouse AML
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Mar 30, 2020 |
| Last update date |
Dec 21, 2021 |
| Contact name |
Takuro Nakamura |
| E-mail(s) |
takuro-ind@umin.net
|
| Phone |
81-3-3570-0462
|
| Organization name |
Japanese Foundation for Cancer Research
|
| Department |
The Cancer Institute
|
| Lab |
Carcinogenesis
|
| Street address |
3-8-31 Ariake, Koto-ku
|
| City |
Tokyo |
| ZIP/Postal code |
135-8550 |
| Country |
Japan |
| |
|
| Platform ID |
GPL11180 |
| Series (2) |
| GSE147797 |
BCL11A promotes myeloid leukemogenesis by abrogating the transcriptional activity of PU.1 [array] |
| GSE147798 |
BCL11A promotes myeloid leukemogenesis by abrogating the transcriptional activity of PU.1 |
|
