|
Status |
Public on Oct 02, 2009 |
Title |
IR-Pv/Vt-1 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
IR-Pv-1
|
Organism |
Solea senegalensis |
Characteristics |
tissue: ovary pieces developmental stage: previtellogenic
|
Growth protocol |
Females were sacrificed at different times during the annual reproductive cycle and pieces of the ovary were deep-frozen
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from previtellogenic and vitellogenic ovaries using the RNeasy extraction kit (Qiagen) and treated with DNAse following the manufacturer’s instructions
|
Label |
Cy5
|
Label protocol |
Total RNA (0.5 μg) from each sample was amplified and labelled with fluorescent cyanine dyes, Cy3 or Cy5, using the Eberwein mRNA amplification procedure employing the MessageAmp™ aRNA amplification kit from Ambion (Applied Biosystems) following the manufacturer's instructions with minor modifications
|
|
|
Channel 2 |
Source name |
IR-Vt-1
|
Organism |
Solea senegalensis |
Characteristics |
tissue: ovary pieces developmental stage: vitellogenic
|
Growth protocol |
Females were sacrificed at different times during the annual reproductive cycle and pieces of the ovary were deep-frozen
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from previtellogenic and vitellogenic ovaries using the RNeasy extraction kit (Qiagen) and treated with DNAse following the manufacturer’s instructions
|
Label |
Cy3
|
Label protocol |
Total RNA (0.5 μg) from each sample was amplified and labelled with fluorescent cyanine dyes, Cy3 or Cy5, using the Eberwein mRNA amplification procedure employing the MessageAmp™ aRNA amplification kit from Ambion (Applied Biosystems) following the manufacturer's instructions with minor modifications
|
|
|
|
Hybridization protocol |
Hybridizations were carried out for 17 h at 60°C using Agilent's gaskets G2534-60002, G2534A hybridization chambers, and DNA Hybridization Oven G2545A, according to the manufacturer's instructions
|
Scan protocol |
Microarray raw data were obtained using Agilent's DNA Microarray Scanner G2505B and Feature Extraction software (v10.1)
|
Description |
Biological replicate 1 of 3
|
Data processing |
The raw fluorescence intensity data were processed using the Polyphemus™ software (Oryzon Genomics), Q-Splines normalization.
|
|
|
Submission date |
Jul 27, 2009 |
Last update date |
Oct 02, 2009 |
Contact name |
angela tingaud-sequeira |
E-mail(s) |
angela.tingaud@u-bordeaux1.fr
|
Organization name |
Université Bordeaux 1
|
Lab |
GPP
|
Street address |
avenue des Facultés
|
City |
Talence |
ZIP/Postal code |
33400 |
Country |
France |
|
|
Platform ID |
GPL8931 |
Series (2) |
GSE17336 |
Oogenesis in Solea senegalensis: Previtellogenic versus vitellogenic ovaries |
GSE17337 |
Oogenesis in Solea senegalensis: various developmental stages |
|