|
| Status |
Public on Jun 25, 2009 |
| Title |
retina_wt_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
retina wildtype
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Retina
age: Postnatal day 4
genotype: mixed C57/129
|
| Treatment protocol |
Retinas were transferred to RNase free microtubes and snap frozen on dry ice
|
| Growth protocol |
Neonatal mice were euthanized at P4, eyes were enucleated and retinas dissected out
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the retinas using the Qiagen RNeasy Micro Kit according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2ug total RNA (Expression Analysis Technical Manual, 2005-2009, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA was hybridized for 16 hr at 45C on GeneChip MOE 430A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
retina_wt_rep1
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Jun 23, 2009 |
| Last update date |
Jun 24, 2009 |
| Contact name |
David Arthur Simpson |
| E-mail(s) |
david.simpson@qub.ac.uk
|
| Organization name |
Queen's University Belfast
|
| Department |
Wellcome-Wolfson Institite for Experimental Medicine
|
| Street address |
The Wellcome – Wolfson Institute for Experimental Medicine, 97 Lisburn Road
|
| City |
Belfast |
| State/province |
N. Ireland |
| ZIP/Postal code |
BT9 7BL |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE16756 |
Expression data from retinas of wildtype and Nhlh2 knockout mice |
|
