|
| Status |
Public on Feb 15, 2010 |
| Title |
TM 86 treated with FA at 1uM, rep A |
| Sample type |
RNA |
| |
|
| Source name |
TM 86 treated with FA at 1uM
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: human trabecular meshwork cells
cell line: TM 86
|
| Treatment protocol |
TM 86 and TM 93 cells were treated with Dex, FA, and TA at 1 uM or 0.1% DMSO v/v as vehicle control .
|
| Growth protocol |
TM 86 and TM 93 cells were maintained in low glucose DMEM medium supplemented with 10% FBS and antibiotics.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from flash frozen cell culture plates using RNeasy Mini columns (Qiagen, Valencia, CA) according to the manufacturer's protocol. There were three biological replicates per treatment condition.
|
| Label |
Cy3
|
| Label protocol |
500 ng of total RNA from each RNA sample was amplified and labeled with a fluorescent dye (Cy3) using the Low RNA Input Linear Amplification Labeling kit (Agilent Technologies, Palo Alto, CA, USA) following the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
An equal amount of Cy3-labeled cRNA (1.6 g) was hybridized to the Agilent Human Whole Genome Oligo Microarray (Agilent Technologies) for 17 hours, prior to washing and scanning.
|
| Scan protocol |
Data were extracted from scanned images using Agilent's Feature Extraction Software (Agilent Technologies) using default settings.
|
| Description |
2/8/08 TM 86 p5 FA A 24 hr
|
| Data processing |
Gene expression data were loaded into the Rosetta Resolver® Gene Expression Analysis System version 7.1.0.1.11. Data from three biological replicate hybridizations were combined using an error-weighted average and the following criteria was used to identify differentially expressed transcripts: a log ratio p-value < 0.001, and a log(10) intensity measurement > -1.8.
|
| |
|
| Submission date |
Jun 16, 2009 |
| Last update date |
Feb 15, 2010 |
| Contact name |
Alissar Nehme |
| E-mail(s) |
nehme_alissar@allergan.com
|
| Organization name |
Allergan Inc.
|
| Street address |
2525 Dupont Drive
|
| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92612 |
| Country |
USA |
| |
|
| Platform ID |
GPL6480 |
| Series (1) |
| GSE16643 |
Effect of glucocorticoid chemical structure and receptor potency on human trabecular meshwork cells |
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