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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 15, 2010 |
| Title |
Effect of glucocorticoid chemical structure and receptor potency on human trabecular meshwork cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Glucocorticoids with different chemical structures but similar glucocorticoid receptor potency regulate subsets of common and unique genes in human trabecular meshwork cells. Gene expression changes of human trabecular meshwork cells, TM 86 and TM 93, due to treatment with dexamethasone (Dex), fluocinolone acetonide (FA), and triamcinolone acetonide (TA).
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| Overall design |
Agilent Human Whole Genome Expression oligonucleotide microarray. Changes of genome-wide mRNA transcription levels were determined by treating cultured TM 86 and TM 93 cells with 1 uM of either Dex, FA, and TA for 24 hours versus DMSO vehicle controls. This was followed by total RNA extraction, labeling of RNA with Cy3, and hybridization of Cy3-labeled cRNA to the Agilent Human Whole Genome microarray for 17 hours. The microarray experiment was repeated three times.
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| Contributor(s) |
Nehme A, Lobenhofer EK |
| Citation(s) |
19744340 |
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| Submission date |
Jun 16, 2009 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Alissar Nehme |
| E-mail(s) |
nehme_alissar@allergan.com
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| Organization name |
Allergan Inc.
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| Street address |
2525 Dupont Drive
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| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92612 |
| Country |
USA |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (23)
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| GSM417729 |
TM 93 treated with TA at 1uM, rep C |
| GSM417730 |
TM 93 treated with 0.1% DMSO, rep C |
| GSM417731 |
TM 86 treated with TA at 1uM, rep B |
| GSM417732 |
TM 93 treated with 0.1% DMSO, rep B |
| GSM417733 |
TM 93 treated with TA at 1uM, rep A |
| GSM417734 |
TM 86 treated with 0.1% DMSO, rep B |
| GSM417735 |
TM 86 treated with FA at 1uM, rep B |
| GSM417736 |
TM 86 treated with TA at 1uM, rep C |
| GSM417737 |
TM 86 treated with 0.1% DMSO, rep C |
| GSM417738 |
TM 86 treated with 0.1% DMSO, rep A |
| GSM417739 |
TM 86 treated with TA at 1uM, rep A |
| GSM417740 |
TM 93 treated with 0.1% DMSO, rep A |
| GSM417741 |
TM 93 treated with Dex at 1uM, rep C |
| GSM417742 |
TM 86 treated with FA at 1uM, rep C |
| GSM417743 |
TM 86 treated with FA at 1uM, rep A |
| GSM417744 |
TM 93 treated with Dex at 1uM, rep A |
| GSM417745 |
TM 93 treated with Dex at 1uM, rep B |
| GSM417746 |
TM 93 treated with FA at 1uM, rep C |
| GSM417747 |
TM 86 treated with Dex at 1uM, rep B |
| GSM417748 |
TM 93 treated with FA at 1uM, rep A |
| GSM417749 |
TM 93 treated with FA at 1uM, rep B |
| GSM417750 |
TM 86 treated with Dex at 1uM, rep C |
| GSM417751 |
TM 86 treated with Dex at 1uM, rep A |
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| Relations |
| BioProject |
PRJNA116225 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE16643_RAW.tar |
204.5 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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