|
| Status |
Public on Sep 16, 2009 |
| Title |
breast cancer sample from patient BC135 |
| Sample type |
genomic |
| |
|
| Source name |
patient BC135
|
| Organism |
Homo sapiens |
| Characteristics |
histological grade: 3
tnm: T2pN0
er: positive
pr: positive
her2: NA
invasion: yes
node: negative
histopathology type: invasive breast carcinoma
|
| Treatment protocol |
None
|
| Growth protocol |
None
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from tumor samples using the QIAamp DNA mini kit (Qiagen, Inc., Valencia, CA, USA).
|
| Label |
Biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
As per manufacturer (Affymetrix)
|
| Scan protocol |
Arrays were washed and stained in the Genechip Fluidics Station 450 (Affymetrix) following the manufacturer’s instructions. Chips were scanned with the Affymetrix GeneChip Scanner 3000.
|
| Description |
Hybridized to SNP5.0
primary data: Affymetrix *.CHP files for the 250K_Nsp and 250K_Sty microarrays; Affymetrix *.CHP files for the SNP5.0 microarray.
|
| Data processing |
*.CHP files for the 250K_Nsp and 250K_Sty experiments were generated using the Affymetrix Power Tools version 1.8.5 gtype-probeset-genotype program. *.CHP files for the SNP5.0 experiments were generated using the Affymetrix Power Tools version 1.10.0 apt-probeset-genotype program.
|
| |
|
| Submission date |
Jun 15, 2009 |
| Last update date |
Sep 16, 2009 |
| Contact name |
Robert J Clifford |
| E-mail(s) |
clifforr@mail.nih.gov
|
| Phone |
301-435-1527
|
| Fax |
301-480-6641
|
| Organization name |
NCI
|
| Department |
CCR
|
| Lab |
LPG
|
| Street address |
2115 E Jefferson
|
| City |
Rockville |
| State/province |
MD |
| ZIP/Postal code |
20850 |
| Country |
USA |
| |
|
| Platform ID |
GPL6804 |
| Series (1) |
| GSE16619 |
Identification of novel gene amplification events in breast cancer |
|
