|
| Status |
Public on Nov 08, 2019 |
| Title |
Expt 1 - CD34(A)-D21 |
| Sample type |
RNA |
| |
|
| Source name |
culture
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: cultured CD34+ cells
|
| Treatment protocol |
no treatments were performed, cells in their resting state after preparation
|
| Growth protocol |
2 forms of preparation: CD34+ cells were cultured in StemPro+supplement or basophils, eosinophils and plasmacytoid dendritic cells were purified from the same donor, same blood draw
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mRNA was isolated by miRNEasy kit extractions and contained both mRNA and miRNA, RIN values > 6 (lower allowed because of presence of miRNA)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
the sample title describes a preparation of cells (Expt xx) and the conditions of treatment and follow naming consistent with the published report
mRNA & miRNA
|
| Data processing |
due to the way data was processed in the published description of these results (no global normalization), the matrix of results that is normally entered as normalized is not normalized but instead a replicate of the non-normalized data
|
| |
|
| Submission date |
Oct 08, 2019 |
| Last update date |
Nov 08, 2019 |
| Contact name |
Donald Welton MacGlashan, Jr. |
| E-mail(s) |
dmacglas@jhmi.edu
|
| Phone |
4105502145
|
| Organization name |
Johns Hopkins University
|
| Department |
Allergy and Clinical Immunology
|
| Street address |
5501 Hopkins Bayview Circle
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21224 |
| Country |
USA |
| |
|
| Platform ID |
GPL6883 |
| Series (2) |
|
