|
| Status |
Public on Nov 08, 2019 |
| Title |
Human leukocyte expression profiles (in vitro) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
CD34+ progenitor cells were cultured for 0 or 21 days in StemPro medium + supplement + IL-3 at 5 ng/ml
Basophils, eosinophils and plasmacytoid dendritic cells were purified to near homogeneity and lysed for miRNA and mRNA. Basophils were purified by negative selection (StemSep Abs, miltenyi columns), eosinophils by StemSep Abs and pDC by positive selection after removing monocytes. The method starting with a 2-step Percoll gradient that put eosinophils in the pellet, basophils in the middle layer and pDC in the upper layer.
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| |
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| Overall design |
Cells were isolated from purified to high homegeneity (99%) and lysed for RNA.
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| |
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| Contributor(s) |
MacGlashan D |
| Citation(s) |
31629804 |
| Submission date |
Oct 08, 2019 |
| Last update date |
Nov 08, 2019 |
| Contact name |
Donald Welton MacGlashan, Jr. |
| E-mail(s) |
dmacglas@jhmi.edu
|
| Phone |
4105502145
|
| Organization name |
Johns Hopkins University
|
| Department |
Allergy and Clinical Immunology
|
| Street address |
5501 Hopkins Bayview Circle
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21224 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6883 |
Illumina HumanRef-8 v3.0 expression beadchip |
|
| Samples (21)
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA576503 |
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