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Sample GSM409116 Query DataSets for GSM409116
Status Public on Mar 18, 2010
Title MM - Normal FISH - p1320
Sample type RNA
 
Source name Bone marrow, MM
Organism Homo sapiens
Characteristics tissue: bone marrow
cell type: CD138-positive plasma cell
disease: multiple myeloma
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from normal and tumor plasma cells using miRNEasy Mini Kit (Qiagen, Valencia, USA) following manufacturer's protocol. The RNA integrity was assessed using Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA).
Label biotin
Label protocol Labelling and hybridizations were performed according to protocols from Affymetrix. Briefly, 100-300 ng of total RNA were amplified and labeled using the WT Sense Target labelling and control reagents kit (Affymetrix Inc., Santa Clara, CA, USA)
 
Hybridization protocol Labeled RNA was hybridized to Human Gene 1.0 ST Array
Scan protocol Washing and scanning were performed using GeneChip System of Affymetrix (GeneChip Hybridization Oven 640, GeneChip Fluidics Station 450 and GeneChip Scanner 7G).
Description MM - Normal FISH - p1320
Data processing Expression value for each probe set was calculated using RMAExpress program that uses RMA (Robust Multi-Array Average) algorithm.
 
Submission date May 27, 2009
Last update date Mar 17, 2010
Contact name Norma Carmen Gutierrez
E-mail(s) normagu@usal.es
Phone 34923291375
Organization name University Hospital
Department Hematology
Street address
City Salamanca
ZIP/Postal code 37007
Country Spain
 
Platform ID GPL6244
Series (1)
GSE16558 MicroRNAs in Myeloma

Data table header descriptions
ID_REF
VALUE RMA-normalized expression value

Data table
ID_REF VALUE
7896736 4.199589
7896738 2.534102
7896740 3.046114
7896742 5.080813
7896744 3.538626
7896746 9.326121
7896748 8.749238
7896750 3.053846
7896752 8.651732
7896754 4.029559
7896756 4.369369
7896759 5.983274
7896761 6.24908
7896779 6.368912
7896798 6.230302
7896817 6.615047
7896822 5.574231
7896859 4.655951
7896861 3.45161
7896863 5.324128

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.




Supplementary file Size Download File type/resource
GSM409116.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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