|
| Status |
Public on Nov 25, 2020 |
| Title |
MSC.SM5 |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
disease state: smoldering myeloma
|
| Treatment protocol |
MSCs were recovered by trypsinization, washed once in PBS and stored at -20°C in RLT buffer (Qiagen) until RNA extraction.
|
| Growth protocol |
Primary MSCs from bone marrow (BM) samples of healthy donors (n=8), MGUS (n=10), SMM (n=10) and MM patients (n=24) were generated as described by Garayoa et al. 2009 (PMID:19357701). Ficoll-Paque density gradient separation medium was used to isolate mononuclear cells from BM aspirates. MSCs were selected by adherence to plastic and subsequently expanded until passage 2 when they were used for experiments.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolation, labelling and microarray hybridization was done following the manufacturer’s protocols for the GeneChip platform: Human Gene 1.0 ST oligonucleotide arrays (Affymetrix).
|
| Label |
biotin
|
| Label protocol |
Labeling and hybridization were performed according to protocols from Affymetrix.
|
| |
|
| Hybridization protocol |
Hybridized to the Affymetrix GeneChip Human Gene 1.0 ST oligonucleotide microarray, after quality checking on GeneChips Test Arrays.
|
| Scan protocol |
Labeling and hybridization were performed according to protocols from Affymetrix.
|
| Description |
Gene expression data from human clinical samples
|
| Data processing |
R studio
|
| |
|
| Submission date |
Sep 12, 2019 |
| Last update date |
Nov 26, 2020 |
| Contact name |
Esteban Ballestar |
| Organization name |
Josep Carreras Research Institute (IJC)
|
| Lab |
Epigenetics and Immune Disease
|
| Street address |
Ctra de Can Ruti, Camí de les Escoles s/n
|
| City |
Badalona, Barcelona |
| State/province |
N/A = Not Applicable |
| ZIP/Postal code |
08916 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE137369 |
Targeting aberrant DNA methylation in mesenchymal stromal cells as a treatment for myeloma bone disease [expression] |
| GSE137419 |
Targeting aberrant DNA methylation in mesenchymal stromal cells as a treatment for myeloma bone disease |
|
