|
| Status |
Public on Apr 26, 2010 |
| Title |
glioma 294 |
| Sample type |
RNA |
| |
|
| Source name |
brain tissue, glioma
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: brain
gender: Male
histology: GBM (grade IV)
age at diagnosis: 66.80
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
Gene expression analysis.
|
| Data processing |
Expression levels of 17,527 genes were extracted from Affymetrix HU133 Plus 2.0 arrays using updated array annotation (Dai et al., 2005) based on reorganizing probes to gene specific probe sets, excluding inaccurate or wrongly annotated probes.
|
| |
|
| Submission date |
May 19, 2009 |
| Last update date |
Apr 26, 2010 |
| Contact name |
Pim French |
| E-mail(s) |
p.french@erasmusmc.nl
|
| Phone |
+31 10 70443 33
|
| Organization name |
Erasmus MC
|
| Department |
Neurology
|
| Lab |
Neurooncology
|
| Street address |
Dr Molewaterplein 50
|
| City |
Rotterdam |
| ZIP/Postal code |
2040CA |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL8542 |
| Series (1) |
| GSE16011 |
Intrinsic Gene Expression Profiles of Gliomas are a Better Predictor of Survival than Histology |
|
